Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Is there any possibalities of PDMS coating on Biochar Powder?

Hello, I'm doing a research regarding gas adsorption on hydrophoobic biochar. Is there any way to coat PDMS on the biochar powder?

05 October 2020 5,610 1 View

Phase index and group index of SOI waveguide?

How do I experimentally obtain the group index or phase index of waveguides? What kind of waveguide could help? Any literatures?

29 April 2019 4,391 4 View

Phase index and group index of SOI waveguide?

How do I experimentally obtain the group index or phase index of waveguides? What kind of waveguide could help? Any literatures?

03 April 2019 8,932 6 View

Has anyone done Gibson Assembly to build a large DNA construct?

Dear all, I am currently trying to do a 3-way Gibson Assembly using 3 DNA fragments of size, 2.4 kb, 4 kb and 6.3 kb. The final ligated product will have a size of 12 kb. My protocol involves...

08 January 2018 4,181 5 View

Do you know where to obtain the Bio-Rad MyCycler Gradient Upgrade Kit #1709707EDU?

14 September 2017 7,773 3 View

Is store-bought glycerin (glycerol) USP 99.5% suitable for use as a bacterial cryoprotectant?

It's my guess that this is no different from the glycerol sold by lab supply companies but I was curious to see if anyone has tried using store-bought glycerin to freeze bacteria for cryo-storage...

07 September 2017 3,958 4 View

Publication on semiconductor Q switched laser?

Anyone has any semiconductor Q switched laser to share with me?

07 August 2017 6,315 1 View

Wet Etching of P-GaP?

Any one know of any chemical mixtures that can wet etch P-doped GaP?

26 December 2016 8,788 1 View

Wet Etching of P-GaP?

Any one know of any chemical mixtures that can wet etch P-doped GaP?

11 December 2016 3,772 1 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View