I am a final year student in Malaysia and I am currently working on bioinformatics of TSG 101 in Colorectal Cancer. I would like to ask if anyone has TSG101 sequences in CRC or any CRC cell lines? Thank you!
CIR 2021/808 written as "the CCα shall be calculated as follows: Method 1: by the calibration curve procedure according to ISO 11843-1:1997 ( 14) (here referred to as critical value of the net...
13 May 2024 9,481 0 View
Hello everyone, Thank you for your time and patience in advance! I attempt to develop a pedagogical framework and a competence scale for my PhD project. I have developed the drafts of both. I...
06 May 2024 2,239 3 View
Anyone has test setup to measure silicon photonics modulator with PAM4 200Gbps/lane?Looking for collaboration or paid service. Thanks.
18 April 2024 3,443 0 View
I wonder if anybody knows where I could find and download some sector scan ultrasound images? I need them for doing my homework.
07 April 2024 1,764 1 View
Recently, I was working on cell biology of spinal ligement tissues, the human samples obtained from spinal surgery were easy to identify, but how can I make sure of the isolated spinal tissues...
02 April 2024 2,674 0 View
Assuming there's no detected contamination in the cabinet, how often should I sterilize the CO2 cabinet? Co-worker suggested we should maintain an absolute clean surroundings (to the...
18 March 2024 5,900 0 View
I mapped in Arcgis, where green is the study species and red is the non-study species, how do I analyse the degree of mixing and the angular scale of the species?
12 March 2024 2,548 1 View
I'm currently working with animal tissue, more specifically visceral fat tissues from dogs. Our labs are not equipped to function 24/7 at the moment so I have to make sure the sample collection...
25 February 2024 2,835 4 View
The button to upload one article (privately) is not working for me. Any ideas?
09 February 2024 8,217 0 View
Any suggestion on microarrays (protein/ DNA/ RNA) that measure enzymes in the phospholipid metabolism pathways? Thank you.
18 December 2023 1,365 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 640 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am doing a study on nursing students satisfaction on the hospital attachment whether they are satisfied with the clinical attachment or not. I need more research studies on this
03 August 2024 6,985 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View