How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Best method for immunostaining FLAG-tagged LRRK2 in rodent brain?

I have been carrying out IHC to stain FLAG in fresh-frozen rat brain using FLAG M2 (F1804) with ABC then DAB, but am unable to identify positive staining, and seeing a high background. I carried...

08 October 2020 8,986 2 View

Why Leucine is used for bacterial production measurement?

Leucine uptake assay is widely used for bacterial production. But I have three concerns (1) Why Leucine (not other amino acids) is used and (2) In case of environmental samples, Leucine may be...

25 February 2020 7,758 6 View

Can anyone provide me the literature associated with Leucine Amino Peptidase (LAP) Assay, (Adamor etal, 1963) ?

Leucine Amino Peptidase (LAP) Assay protocol

11 December 2019 7,021 3 View

How to elute the proteins completely after immunoprecipitation?

Dear All, I am using Anti-Flag magnetic beads for immunoprecipitation experiments. I want to perform elution under the non-denaturing conditions for further use of proteins as well as to reuse...

10 November 2019 4,806 7 View

How can I store p-dimethylaminocinnamaldehyde solution, l-leucine b-naphthylamide solution and p-naphthylamine stock solution?

Hello, The aforementioned solutions are to be used for the purpose of conducting soil enzyme assays. Because I will be using the solutions frequently in the coming weeks, I plan to store them....

19 February 2019 6,589 7 View

Leucine 60 to proline mutation in GFPmut3?

We wanted to tag a protein of interest with GFP mut3 version. upon generating the construct and sequencing it we found it has an additional mutation. A conserved leucine at 60th position had got...

07 August 2018 7,631 0 View

Isoleucine and Leucine as equivalent in peptide sequence in uniprot?

May I know is there any significance in treating Isoleucine and Leucine as equivalent in peptide sequence searches? Does it affect the results of the search in finding the 'right' protein if we...

03 April 2018 2,342 0 View

How could alanine substitution vs deletion have an impact on a protein?

I performed deletion mutagenesis along the c terminal region of a protein targeting two specific leucine residues, and showed no change compared to wild type, as assayed by dual...

05 February 2018 4,380 2 View

How could alanine substitution vs deletion have an impact on a protein?

I performed deletion mutagenesis along the c terminal region of a protein targeting two specific leucine residues, and showed no change compared to wild type, as assayed by dual...

01 February 2018 2,611 4 View

Does anybody know an enzyme (aminopeptidase) that cleaves the dipeptide L-alanyl-L-glutamine into glutamine? If so, is it specific?

(We need it to measure L-alanyl-L-glutamine concentrations in a tissue engineering bioreactor.) Thanks, Martin

09 October 2017 3,744 1 View