Hi everyone,
I'm looking for a DNA extraction kit or some protocols to obtain the total DNA from resin samples. For this purpose, some studies suggest the use of plant extraction protocol of famous kit (such as Wizard Genomic kit by Promega).
Thanks,
Dominique
Yes, QIAGEN resin, is unique, anion-exchange resin based on the interaction between negatively charged phosphates of the DNA backbone and positively charged DEAE groups on the surface of the resin. Which eliminates the need for expensive equipment and reagents such as ultracentrifuges, HPLC/FPLC‚ or CsCl. Toxic and mutagenic substances such as phenol, chloroform, and ethidium bromide are also not required. The key advantage of QIAGEN anion-exchange resin arises from its exceptionally high charge density. The resin consists of defined silica beads with a particle size of 100 µm, a large pore size, and a hydrophilic surface coating. The large surface area allows dense coupling of the Diethylaminoethyl groups. Plasmid DNA remains tightly bound to the DEAE groups over a wide range of salt concentrations. Impurities such as RNA, protein, carbohydrates, and small metabolites are washed from QIAGEN resin with medium-salt buffers. The conventional anion-exchangers, based on cellulose, dextran, or agarose, have separation ranges only up to 0.4 M salt, in contrast QIAGEN resin is extremely broad, extending from 0.1 M to 1.6 M salt, and DNA can be efficiently separated from RNA and other impurities. Thus, the separation and purification qualities of QIAGEN resin, as well as its ease of use surpass those of conventional anion-exchange resins. For prize please check the link
https://www.sigmaaldrich.com/catalog/product/sigma/na2110?lang=en®ion=IN&gclid=Cj0KCQjw59n8BRD2ARIsAAmgPmJ_g1bbFgWxJG-UOMjFcxye2kOkBv9TcknDEFZtdm1jYEvBWntjLfIaAojcEALw_wcB
Please look at the following below links which may help you in your analysis:
https://www.thermofisher.com/content/dam/LifeTech/global/life-sciences/DNARNAPurification/Files/0416/DNA-Purification.pdf
https://phagesdb.org/media/workflow/protocols/pdfs/Promega_Wizard_Kit_PDF.pdf
Thanks
- Badges
- Science topic
- Similar topics
- Analytical Chemistry
- Extraction