I want to work on in-vitro studies of a diabetic model. Is it possible? Any cell lines available?
Hi Arbind,
I have one paper with me that details about the cell lines from various species for diabetic studies. Hope this helps you.
Good Luck.
with regards , thanks a lot, its really helpful.
Hi... MIN6 is the best cell line to work in diabetic models. Next to that INS-1E is the another best cell line since it is more sensitive to glucose than other cell lines. INS-1E is the modified cells of INS-1.
thanks with regards
Two independent variables 1. Attachment style 1 to 5 likert scale Strongly disagree to Strongly agree 2. Family functioning 1 to 4 likert scale Strongly agree to strongly disagree 3. Childhood...
10 February 2024 6,384 2 View
while using the TRIZOL RNA extraction method. In the precipitation step using isopropanol, polysaccharides like pectin and xyloglucan also get precipitated along with RNA, hindering the following...
30 January 2024 1,390 0 View
1. http://www.libraweb.net/riviste.php?chiave=114 2. http://tbf.peerjournals.net/ Which one is original
26 December 2023 2,961 1 View
Proteins expressed in pPcZaA for protein purification using his trap column in FPLC? how to prepare a sample for that. We have lyophilized the supernatant at 24 hr, 48 hr and run SDS.
25 December 2023 9,012 0 View
please define steps how to upload research paper
27 November 2023 6,287 2 View
Hi everyone, We use DAMASK 3.0. We want to do dwell fatigue simulations. Can we use phenomenological power law for dwell fatigue/creep simulations? Which Model is best for dwell fatigue/creep...
17 October 2023 3,632 0 View
I am trying to validate the detection of plant fungal pathogen through LAMP reaction. But I have an issue with the LAMP reaction. I am detecting the band in NTC (Control) also. To rectify the...
10 October 2023 1,525 2 View
In exothermic reactions, the rate constant decreases on increasing the temperature. When we plot the graph between ln k and 1/T, a positive slope is obtained which gives negative activation...
21 August 2023 372 1 View
How can the Thermogravimetric analysis (TGA) be used to compare similar products?
19 August 2023 3,742 1 View
Could anyone tell how can the TGA (Thermogravimetric analysis) be used to reverse engineer a product?
19 August 2023 9,667 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View