14 May 2023 0 8K Report

Can the perfusion be performed with any other alternatives? Preferably a low cost option.

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Could someone please provide a list of journals that accept the application of methodologies in nature-based solutions?

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About mineral exploration?

Are there any techniques at ore exploration that could replace the compass-tape measure of mineralized structures?

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Find a full text article?

Hello. I can´'t find a full text article that i need for my research. Can you help me? The reference of the article is: Ref ID: 3441009. Article: Adolescent Health: Communication With Adolescent...

16 May 2024 3,293 3 View

What is the minimum amount of liquid and beads I can use for bead-beating in a 2ml vial?

I am planning to extract DNA with QIAamp Mini DNA extraction kit (Qiagen) from DNA Shield microbiome preserved samples. My samples contain small amount of cell, so my goal is to reduce the loss of...

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Can I replace some of the buffers from the first steps of QIAamp Mini kit by DNAshield?

I am planning to extract DNA with QIAamp Mini DNA extraction kit (Qiagen) from DNA Shield microbiome preserved samples. My samples contain small amount of cell, so my goal is to reduce the loss of...

14 May 2024 7,178 3 View

How to properly extract proteins from a Caco-2 cell line?

Hi everyone! As I previously shared with you, I've started to work with Caco-2 cells and even asked for your help. I recently followed a protocol to extract proteins, but when I collected the...

12 May 2024 6,819 2 View

How do I measure the virulence intensity of a fungus strain in a host plant?

I need to determinate how virulent is a fungus/oomycete strain-specie in comparative with others. I have plants group with and without fungus symptoms. However I found the fungus sources in both...

06 May 2024 8,974 5 View

How long do human brain tissue samples last after protein isolation?

Hello! I am currently running a western blot using human AD tissue samples. I prepared these samples over a year and a half ago. I am having trouble standardizing these proteins with Beta Actin. I...

02 May 2024 9,588 3 View

How to get successful bacterial plasmid growth?

I am currently running a plasmid isolation. I am using a bacterial stab to grow the plasmids. We have used this stab a few times now. Unfortunately, we have not had the chance to make glycerol...

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Any protocol for loading dsRNA in an agarose gel?

I am expressing dsRNA using E.coli as vector. I would like to check that my bacteria culture is expressing dsRNA. I am planning to extract RNA from my bacteria culture and then load the total RNA...

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