Sir, During transcription a double helix of DNA unwinded only for the very small region and winded again when the RNA polymerase going forward. The hydrogen bond is basically to keep attachment of two strands of DNA. In case of RNA they are mostly single strand so need of hydrogen bond.
Dear sir ,thank you for answer but my question about the transcrption process , during it are the developed RNA form hydrogen bond with template DNA for short time before it migration away from DNA strand
That is how bases are selected, by Watson-Crick base pairing. The DNA-RNA hybrid formed during transcription is same as a double stranded DNA molecule, the only difference being the presence of uracil (U) instead of thymine (T) in the RNA strand. This hybrid is formed over a small stretch and progresses with extension from 5' to 3' , as unwinding occurs simultaneously to release a single stranded RNA molecule.
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