How to customize a 'plt.var' plot from CCA package?

Hello, I'm trying to customize a CCA biplot made with the plt.var function from the CCA package. I need to reduce the size of the labels or be able to move those labels because they overlap on...

31 July 2020 2,938 3 View

Unexpected results when using MitoTracker Green FM and CCCP treatment in cytometry. How to explain?

We want to evaluate mitochondrial mass. Cells were stained with MitoTracker Green FM (Cat. M7514), whose fluorescence monitors total mitochondrial mass and in particular, we want to study...

22 November 2018 3,151 2 View

Any research connecting muscle mass and ventilation/breathing frequency in resting conditions?

As distance runners have a lower muscle mass but they involve bigger muscle mass than some other disciplines. What is the impact on that issue over respiratory parameters like ventilation and...

11 May 2017 2,021 3 View

Does anybody know the diameter size of mitochondria isolated from rat heart?

07 February 2016 7,142 4 View

Which is the best animal model for studying utricle and sacule?

Which is the best animal model for studying utricle and sacule? We are specially interested in cell culture and we found some dificulties in extracting utricule cells from mouses

02 February 2015 7,470 3 View

Which is the best animal model for studying utricle and sacule?

Which is the best animal model for studying utricle and sacule? We are specially interested in cell culture and we found some dificulties in extracting utricule cells from mouses

01 February 2015 886 3 View

Is there any goodness of fit test (different from the Anderson-Darling test) that is better than the Kolmogorov-Smirnov test?

I'm currently teaching Statistics for Civil Engineerings and I would like to know if there is something different from the KS test that is worth to show to my students

11 May 2014 2,213 6 View

What's the most efficient way to purify DNA from an agarose gel?

I did ask for the most efficient (higher recovery, good quality) but not fastest.

04 May 2014 8,560 10 View

Antibody RYR2 Immunoflurescence?

I am trying to make a IF from isolated cardiomyocytes (mouse) for RYR2 but I don't get any pattern. After using several concentrations and protocols I have decided to buy a new antibody. Does...

08 July 2013 4,735 5 View

Antibody RYR2 Immunoflurescence?

I am trying to make a IF from isolated cardiomyocytes (mouse) for RYR2 but I don't get any pattern. After using several concentrations and protocols I have decided to buy a new antibody. Does...

06 July 2013 7,044 6 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

Hello, does any one know a free medical text dataset?

a free multi label clinical text dataset , containing text as entry and several labels as classes. Best regards

22 February 2021 5,988 2 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

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22 February 2021 9,932 11 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View