Are proximity labeling enzymes (BirA, APEX, TurboID...) sensitive to pH? Will they work in very acidic pH (pH 5.5)?

Hi everyone, Has anyone tried using any of the proximity labeling enzymes (BirA, APEX, TurboID...) to biotinylate proteins in a very acidic media (~pH 6)? Will it work? Alice Ting's lab always...

08 August 2018 5,702 1 View