Does anybody know a tool or software to identify unknown point mutations causing resistance in bacteria? I know PointFinder exists but I would prefer a package available in conda. Anyway, all suggestions are welcomed!
I expressed my protein in cell free with wheat germ, and after purification on streptag with (2,5mM, elution) i observed 2 bands on gels at the same weight. I have already expressed the protein...
17 June 2024 5,742 4 View
The effect of the special program in the arts (SPA) curriculum to the completers.
11 April 2024 7,297 1 View
Hello everyone, I'm a master student and in my project I'm studying RFP-based constructs (RFP + few amino acid-long tags). I'm now performing a Western blot in SDS-PAGE of my cell-line lysates....
04 April 2024 7,382 3 View
I have whole insects in RNAlater but I will only use some body parts for the RNA extraction.
20 February 2024 6,537 0 View
This query is related to the group's assigned journal "Tenets of Specimen Management in Diagnostic Microbiology".
07 February 2024 2,355 1 View
Hi! I am trying to analyze a moderated mediation using mplus. I first started with a simple mediation, so X -> M -> Y. Path a (X -> M) is not significant, while Path b (M -> Y) is...
25 January 2024 3,738 2 View
I've been using LUHMES cells to differentiate into dopaminergic neurons for over 6 months now, but i always have issues when it comes to platting for differentiation. It's always an ON/OFF kind of...
21 January 2024 8,780 2 View
Hello, I am looking for technical advices. I have never worked on mouse cells before. I plan to use lipofectamine RNAiMAX as transfection reagent. Can anyone tell me where can I find protocols or...
08 January 2024 2,680 0 View
Dear all, please comment about this. Thanks Letter: Bacteriophages and Colistin: A Promising Therapy and... Anionic Phage followed by cationic antimicrobial peptide Colistin Sequential Therapy...
23 November 2023 9,445 0 View
Hello, I was wondering if it is possible to fix HUVEC cells for IHC and/or TEM analysis after they completed tube formation in Matrigel. I use Corning® Matrigel® Growth Factor Reduced (GFR)...
20 November 2023 5,951 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
IEEE Conference template
02 August 2024 8,762 2 View
Why is nonpoint source pollution potentially more harmful and difference between point and nonpoint sources of water pollution?
01 August 2024 1,180 2 View
How do living organisms play a role in the water cycle and why is nonpoint source pollution potentially more harmful than point source pollution?
01 August 2024 7,061 2 View
How does nonpoint source pollution affect the ocean environment and why point source pollution is often easier to manage than nonpoint source pollution?
01 August 2024 8,014 2 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View
Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.
28 July 2024 6,501 0 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
"Integration of Mass Media and Communication into the System of International Relations: Place, Role, Actuality and Prospects: Modern Media Issues 2024" Website: https://immcir.alnoor.edu.iq/
23 July 2024 3,139 6 View