From my qPCR analysis the values of my negative controls were zero which means there was no contamination as there were no amplifications. Are the negative control values the control values that should be used to calculate the delta delta Ct?
Thanks
In your qPCR experimentation, if you want to calculate the delta delta CT, it means that you want to compare the experimental condition with a control condition (eg : healthy VS disease). You will need 4 points to calculate this : the CT values of the target gene measured in both experimental and control condition, and the CT values of a housekeeping genes measured in both experimental and control (this will calibrate your measure). I assume the control values your talking about refer to the housekeeping gene CT points.
Negative controls are important to verify the absence of exterior contamination.
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