Funmilayo Ajisola

4 Questions 3 Answers 0 Followers

Questions related from Funmilayo Ajisola

How do you write an excellent scientific methods for a project report?

Hello, I am struggling to make the method section of my research work very scientific, so i was wondering if anyone could help with guidelines. The techniques used were SDS-PAGE, Western...

05 May 2017 3,678 1 View

Are my negative controls the controls that i need to use for calculated delta delta Ct?

From my qPCR analysis the values of my negative controls were zero which means there was no contamination as there were no amplifications. Are the negative control values the control values that...

25 April 2017 3,903 1 View

What marker should i use for my SDS PAGE electrophoresis?

Hello All, I am working with HepG2 cell lines and will be using AMPK alpha AB from rabbit and GAPDH AB from rabbit as my primary antibody for western blot. What marker should i use on my SDS-PAGE...

31 March 2017 9,336 3 View

Hello all, i prepared SDS-TANK Buffer (Running Buffer) by using 1g of SDS instead of 10g. Is it possible that i add the remaining 9g to the solution?

Basically i weight out 30.3g of tris, 144.13g of glycine and 1g of SDS instead of weighing 10g of SDS to make up to 1 litre of the buffer with distilled water. Is possible that i go back the next...

28 February 2017 2,602 3 View