How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

What influences inter-day reproducibility in UPLC-MS/MS analysis?

I was analyzing some metabolites in human plasma. While the intraday precision of these metabolites was acceptable ( less than 20%), the interday precision was quite large. So what influences...

13 February 2021 9,183 9 View

A good day to you, Great minds. How do I evaporate hexane and Ethyl acetate extract of bacterial metabolite?

I'm really struggling with it. It takes hours on a water bath at 50 degrees

03 February 2021 1,619 3 View

Why some samples don't give any peaks in HPLC when the rest does?

Hello everyone, Last week we ran an HPLC assay with bacterial supernatant samples. The metabolites measured were SCFA and I used a dilution factor of 2 for some samples and 7 for others (because...

22 January 2021 8,667 6 View

What is the role of pO2 and pCO2 in cell culture?

Using a Nova bioprofile (https://www.fannin.eu/wp-content/uploads/2018/07/BioProfile-Flex.pdf) to sample my CHO culture daily I get various metabolite results and also pO2 and pCO2 results. I am...

13 December 2020 7,998 1 View

How to identify possible metabolite from chromatogram?

We have done the analysis for a pre-clinical study and we have observed additional peaks in the chromatograms. We think that these peaks are metabolites. The concentration of the additional peaks...

02 December 2020 6,134 10 View

What is an efficient way of extracting metabolites for fungal isolate from soil for test on its antimicrobial activity?

Hi, I'm finding a method to extract metabolites to evaluate its antimicrobial activity but the identification of the fungi is not done yet. I'm looking for a universal way to extract the...

24 November 2020 2,273 3 View

What is the significance of serotonin and its metabolite on stress ?

What is the significance of increasing serotonin level of stressed rat compared to normal one and slight increase of 5HIAA level in stressed rat. With higher 5HIAA/5HT ratio in normal one.

21 October 2020 2,165 5 View

How to find compounds that are similar to others in term of structure and activity?

Dear all, I Have found in silico two compounds that are interesting for me, one is spermidine (a metabolite) and another one is negamycin. Negamycin has apparently an effect on my cellular models...

20 October 2020 8,394 3 View

Can anyone suggest me any article which contain specific plant metabolites against antimicrobial activity?

I would like to any lab based plant metabolites which directly interact with multidrug resistant bacteria or any wet lab based molecules able to do the same.

30 August 2020 2,629 4 View

Any suggestions for a simple method to reduce the viscosity of bacterial growth media?

Hi, I am working with a bacteria from the genus Paenibacillus. I am having trouble separating it from the media (PDB), and difficulties in extraction of secondary metabolites from the media,...

04 August 2020 9,974 3 View