I will start an experiment with synthetic antimicrobial peptides against bacteria. I would like to know if we need to get rid of TFA salts. Thank you
Dear Joas,
Since you are going to conduct your experiments at physiological environment of pH 7.4, there is no need to convert the salt to the free acid. At pH 7.4 your peptide will be ionized and it does NOT matter what you starting point is (acid or salt).
The conclusion, there is no need to do any modification of the purchased peptide.
Hoping this will be helpful,
Rafik
Although there might be a concern that trifluoroacetate ion could inhibit bacterial growth if the concentration is high enough, this can easily be checked by testing sodium trifluoroacetate for inhibition of growth.
It is common to test trifluoroacetate salts of compounds for bacterial growth inhibition at concentrations in the micromolar range, with no effect of trifluoroacetate.
If you have the possiblity, you could just re-suspend your peptide in 10 mM HCl and then freeze-dry it. It is not so time-consuming and you would reduce the amount of TFA bound to your peptide.
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