How do you account for the central tendency of mean values in Likert-type response data?

To be more specific, this question does not concern central tendency bias/error -- where respondents are inclined towards centralized responses -- but the concept that mean values expressed on...

24 May 2017 9,238 6 View

Can 2D incompressible, pressure driven flow in a channel (with no-slip BCs) develop a mean velocity component in the wall-normal direction?

17 August 2016 2,165 3 View

How best to grow active osteoclasts?

Hi all, I would like to set up a system for culturing active osteoclasts and I was wondering what people would recommend for a substrate? I know that dentin and bone can be used, but I was...

28 March 2016 4,580 3 View

Does anyone know the maximum haematoxylin & eosin staining time to reach saturation in most tissue sections?

I will be using a variety of tissue sections, and will need to stain them for a range of time periods up until the (hopefully) maximum saturation time of the tissue that takes the longest time to...

01 October 2015 8,199 8 View

What research is being conducted on quantifying in standard units the direct and indirect damage caused to the environment at a micro or macro level by the actual use of natural resources?

All Natural resources are "Free" before we start to make any use of them, and we need to place a value on them by the damage by their use they cause to itself and the rest of the environment,...

13 March 2013 6,672 1 View

Although students have domain knowledge they are still unable to think critically nor solve problems

Solving problems an critically thinking have been at the heart of Educational discussions for hundreds of years. Some believe that solving problems and critically thinking is completely natural...

01 January 1970 1,487 4 View

If visual communication occurs is it translated into visual thinking that insights visual learning?

Does the conversion of visual language into visual communication through visual perception create visual thinking? Does visual thinking create visual learning? Does visual thinking create visual...

01 January 1970 9,067 19 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

What is the cause of the banding obtained after purifying the PCR product with ExoSAPIT?

Hello We are working on a PCR product with an approximate size between 5000 and 7000 bp, we want to use it as a positive internal control, since we have sequenced it and it corresponds to the...

17 February 2021 2,896 3 View