Hello all,

I am confused about calculating the CFU/mL for my serial dilutions, for context I am conducting an experiment that involves creating a standard curve of absorbency (measured via spectrophotometry) against CFU/mL of each dilution. I have conducted the spectrophotometry and plated each of my serial dilutions but my confusion occured when I began calculating the CFU/mL and found that it increased as my samples got more dilute which logically is the opposite of what I expected. Furthermore, I am unsure if it possible to calculate the viable cells in each dilution as the calculation CFU/mL=(number of colonies*dilution factor)/volume plated, seems to be for calculating the number of viable cells in the origional sample.

Any advice would be greatly appreciated.

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