Hello,

I did some flow cytometry acquisition a week ago. Now, I finally got the chance to analyze the data and I unfortunately discovered that one of my compensation controls didn't work well: not enough events were acquired, PMT voltage was set too high, and positive events (dead cells) don't appear (see attached pictures). Therefore, my compensation matrix doesn't make any sense (pictures attached).

I was thinking if it is okay to apply a compensation matrix that is the result of an old acquisition (two months) of compensation controls of the same panel, that worked perfectly? I know for sure that it is not the best thing to do, but giving the circumstances is it a tolerable thing to do? I mean I will for sure have to repeat the experiment and re-do the acquisition, paying more attention to the quality of my compensation controls, but I was hoping to make some use of this data.

Thank you,

Martin

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