Martin Schnitzler

8 Questions 4 Answers 0 Followers

Questions related from Martin Schnitzler

Serum dialysis instead of heat-inactivation for immunometabolism?

Hello, I use heat-inactivated sera for my cell culture experiments. The main goal of the heat-inactivation is to inactivate the complement system. I have now decided that it would be best to...

23 September 2020 3,203 1 View

A decrease in percentage of stained population but the MFI remains relatively unchanged?

Hello, I want to present my cytometry data using histograms/scatter dot plots. I thought I should focus on MFI to export my data. However, in some cases where I see a change in the percentage of...

20 December 2019 4,010 3 View

A decrease in percentage of stained population but the MFI remains relatively unchanged?

Hello, I want to present my cytometry data using histograms/scatter dot plots. I thought I should focus on MFI to export my data. However, in some cases where I see a change in the percentage of...

12 December 2019 4,382 3 View

Applying an old compensation matrix to dataset ?

Hello, I did some flow cytometry acquisition a week ago. Now, I finally got the chance to analyze the data and I unfortunately discovered that one of my compensation controls didn't work well:...

23 September 2019 6,239 4 View

Differences in negative population signal between FMO and unstained controls?

Hello, I wanted to know if someone could explain to me why gating on negative populations of an unstained sample and a FMO control sample is sometimes different? (see screenshorts below) And...

09 September 2019 3,114 5 View

Applying an old compensation matrix to dataset ?

Hello, I did some flow cytometry acquisition a week ago. Now, I finally got the chance to analyze the data and I unfortunately discovered that one of my compensation controls didn't work well:...

09 September 2019 9,680 4 View

Adjusting PMT voltages for lower compensation percentages?

Hello, I have been told that adjusting PMT voltages to lower compensation values is not a good practice. I found this PDF document (attached below) that is supposed to explain the impact of...

05 May 2019 3,741 1 View

FSC and SSC gating for viability dye compensation?

Hello, I am using the LIVE/DEAD™ Fixable Aqua Dead Cell Stain from Thermofisher (https://www.thermofisher.com/order/catalog/product/L34965), to distinguish dead cells. I use it on freshly...

05 May 2019 336 2 View