Has anyone used the Apollo324 kit for miRNA seq experiments? Apparently it works well with small amounts of starting material. I would also like to know if anyone has used a small RNA fraction (
Check out http://www.nature.com/natureevents/science/events/47533-microRNA_Analysis_Using_Next_Generation_Sequencing
I want to collect saliva samples in the Zymo DNA RNA shield in order to extract human DNA and RNA from the saliva. Has anyone tried this, either DNA, RNA or preferably both, and was the quality...
08 September 2019 7,921 1 View
Our inclusion criteria are that participants must be 18 years or older and that they must be able to read and understand English/Afrikaans in order to take part in the study. However, the informed...
02 March 2018 7,114 3 View
Can anyone recommend validated online (web-based) questionnaires to evaluate neuropsychiatric disorders/symptoms?
09 October 2017 542 4 View
I will be visiting Lyon in October and is interested to visit some labs there that preferably work in the behavioural neuroscience/neuro genetics/microbiome/transciptomics/epigenetics fields. Just...
08 September 2014 6,829 5 View
We are planning on doing microbiome analyses with next generation sequencing and would like to know what kits we should use for collection and extraction of microbial DNA?
06 July 2014 5,772 15 View
We are having trouble extracting RNA from Oragene samples; we have very low 260:230 ratios and certain samples have very low concentrations. We have tried the RNAeasy kit, the trizol and a...
01 February 2014 6,228 2 View
I want to make sure if my calculations are correct. I have 370ng/ul stock of my 3'UTR reporter plasmid, I have to use 10ng during the transfection, does that mean that I use 0.03ul of the stock...
07 August 2013 8,525 6 View
I have neuroscreen-1 (NS-1) cells (theses cell are sub-clones from PC12 cells) which I want to co-transfect with a specific miRNA as well as that miRNAs predicted mRNA target, to see if the miRNA...
06 July 2013 7,835 11 View
Which products or brands would you recommend for performing miRNA luciferase assays to determine if an mRNA sequence is a direct target of the particular miRNA? Specifically relating to the miRNA...
05 June 2013 6,443 1 View
I would like to know what prediction programs are out there that predict CpG islands within gene bodies (not promoters).
01 February 2013 8,731 2 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...
24 June 2024 3,507 2 View
Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...
18 June 2024 2,799 4 View
Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...
16 June 2024 9,723 2 View
Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...
06 June 2024 5,715 4 View
The size miRNA is 89 bps and the primers I have designed (using traditional method for designing gene primers) should give an amplified product of 71 bps. But I am getting product of higher...
23 May 2024 7,570 3 View
I have a question regarding target miRNA expression after transfection with antisense miRNA (miRNA inhibitor). I understand that the expression of the target miRNA will increase after mimic...
20 May 2024 5,920 4 View
Hello. Is there any webserver where I can input a set of transcription factors, genes, and miRNAs, and it can provide me with statistical data on how TFs either activate or repress genes and...
18 May 2024 5,848 1 View
Hello all. I have recently been trying to isolate total RNA after transfection of miRNA mimics and antisense inhibitors in mammalian cells. However, the RNA isolated through the trizol method...
13 May 2024 4,935 7 View
would like to work on microRNA to detect colorectal cancer. I will use the type of microRNA type micRNA124,micro143 how I can make primer design for it and how I can choose the primer ?
07 May 2024 8,812 2 View