SW872 cells are a liposarcoma cell line and I am having difficulty with culturing them. I thawed an original vial from ATCC using the media and conditions that was recommended on the data sheet (L-15 media + 10% FBS + Glutamine incubated at 37oC in 0% CO2). The cells initially attached well to the flask and I split them 1:4 for expanded growth to form stocks. Day 1 after the cells were split they were attached to the flask however by day 2 the cells were balling up and floating in the media. Is anyone aware of any particular culturing conditions for this cell line that are not specified by ATCC? I have noticed that the media in the flask is becoming cloudy and I'm assuming that the cells are excreting lipids as they grow, is this detrimental to cell growth overtime?
Any suggests would be appreciated.
Thanks.
Based on the information you shared, it is likely that your culture medium is contaminated. Because these cells are adherent, floating cells are not predicted to "secrete" anything. Therefore, check medium for any contamination.
If you have a fresh vial of cells, seed cells at higher density in a flask and keep the flask lid little loose (for free air exchange). Upon ~80% confluence, split 1:3.
Thanks for your reply Divaker.
The cells were from an original ATCC vial and are the only ones that I have.
Not all of the cells were floaty in the media. There are some that are still attached to the flask. I just thought that it was odd for the cells to be appear happily attached one day and the next day to be balling up and floating.
The media I am using for this cell line is also being used for another cell line under the same incubation conditions and the medium remains clear on this cell line. This is why I thought that it was something that the cells maybe excreting. But maybe the vial of cells from ATCC was contaminated.
Dear Donna,
we are culturing SW872 cells in RPMI 1640 medium supplemented with 10% FBS and 1% Pen./Strep at 37°C in 5% CO2 . For us this works.
Good luck,
Sara
I have worked with other SW cancer cell lines, I used to use RPMI with 155 FCS and i used conditioned medium ( medium that cancer cells grew in for 5 days then drawn and filtered to be added 1:1 to the new medium ). Other thing is you have to add little amount of medium in the first days just to cover the cells.
anyone has experience concerning for how many passages you can culture these cells?
Thanks
stefania
do anyone know how to use oleic acid on this cells (SW872) to induce adipogenesis?
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