Does anyone have a reference for the successful use of CADM1 by immunohistochemistry in formalin-fixed (FFPE) tissue?

I cannot find an antibody for CADM1 that works by immunohistochemistry in human fixed tissue (FFPE). I can find multiple products, but none seem to have been unequivocally validated in humans by...

08 September 2019 1,601 3 View

Has anyone been able to optimise DC-LAMP (LAMP3 / CD208) using immunohistochemistry in formalin-fixed biopsies?

Has anyone been able to optimise DC-LAMP (LAMP3 / CD208) using immunohistochemistry in formalin-fixed biopsies? We have tried several available antibodies for use in human biopsies, but none are...

01 February 2019 2,159 4 View

Quench Alkaline Phosphatase in between 2 consecutive AP-based IHC reactions (double staining) - any ideas?

We are trying double IHC staining with 2 mouse IgG1 monoclonal primaries. In both cases the chromogens (Fast Red & Vector Blue) bind to AP and the secondaries in both reactions are AP-labelled...

02 March 2014 8,466 10 View

How can we minimise / avoid autofluorescence of Eosinophils in Hodgkin tissue?

We are performing multiplexed immunofluorescence on formalin-fixed, paraffin embedded Hodgkin lymphoma. A major issue has been autofluorescence or non-specific binding of what we think are...

10 November 2013 3,992 2 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View