Has anyone been able to optimise DC-LAMP (LAMP3 / CD208) using immunohistochemistry in formalin-fixed biopsies?
We have tried several available antibodies for use in human biopsies, but none are validated in FFPE biopsies (only frozen), and we haven't had any luck with getting them to work.
Mouse / rabbit antibodies (anti-human) ideal.
Thanks
There are a large number of clones directed against CD208. But the best clone against DCLamp, recognized by the entire scientific community, is a Rat monoclonal antibody, clone 1010E1 (from Dendritics). this one works perfectly in paraffin, that the biopsy is fixed in Formalin or in Bouin. This clone works on all animal species, and of course on humans. Ref: DDX0191. This clone is regularly used for its good prognostic value in lung cancer of the smoker.
Sorry, I forgot to mention that the clone DDX 0191 (DClamp 1010E1) is available via NOVUS in England
Jean-Jacques Pin thank you. Yes this is the one I ordered but the anti-rat secondaries don't work in the particular multiplexed project I am trying to optimise. I should have stated this. I really appreciate you taking the time to reply.
hello Chris To perform multiplexing, if the rat clone is a problem, you can use clone 1010E1 directly coupled to biotin or better coupled directly to peroxidase. Otherwise there are many other clones available as 104G4 and 109G3 which are murine antibodies. It is also possible to have these antibodies coupled with fluorophores emitting at different wavelengths: very useful for multiplex assays. If you need help, you can get information via [email protected] Good luck for the future
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