Chris Carey

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Questions related from Chris Carey

Does anyone have a reference for the successful use of CADM1 by immunohistochemistry in formalin-fixed (FFPE) tissue?

I cannot find an antibody for CADM1 that works by immunohistochemistry in human fixed tissue (FFPE). I can find multiple products, but none seem to have been unequivocally validated in humans by...

09 September 2019 1,642 3 View

Has anyone been able to optimise DC-LAMP (LAMP3 / CD208) using immunohistochemistry in formalin-fixed biopsies?

Has anyone been able to optimise DC-LAMP (LAMP3 / CD208) using immunohistochemistry in formalin-fixed biopsies? We have tried several available antibodies for use in human biopsies, but none are...

02 February 2019 2,206 4 View

Quench Alkaline Phosphatase in between 2 consecutive AP-based IHC reactions (double staining) - any ideas?

We are trying double IHC staining with 2 mouse IgG1 monoclonal primaries. In both cases the chromogens (Fast Red & Vector Blue) bind to AP and the secondaries in both reactions are AP-labelled...

03 March 2014 8,519 10 View

Anyone know of a reliable and stable green chromogen?

Performing multiplexed immunohistochemistry on FFPE tissues. Need to find a reliable and robust green chromogen for membranous staining, one that can be colour-deconvoluted from other colour in...

01 January 2014 7,796 0 View

How can we minimise / avoid autofluorescence of Eosinophils in Hodgkin tissue?

We are performing multiplexed immunofluorescence on formalin-fixed, paraffin embedded Hodgkin lymphoma. A major issue has been autofluorescence or non-specific binding of what we think are...

11 November 2013 4,032 2 View