What should be sample size of traning dataset for applying data mining techniques?

21 December 2020 8,119 3 View

Can I combine data from two separate fluorometeic experiments?

I performed a fluorometric experiment with two different cell lines. I had four conditions per experiment, and four "sub-conditions" per condition, wherein I add three different chemicals (the...

08 November 2020 5,113 3 View

Is it possible to calculate Absorption co-efficient or Absorbance of a dye theoretically?

I am working on a Luminescent Solar Concentrators (LSC) using dyes such as Coumarin 1, Coumarin 153, and Perylene Green Ph-probe 720-5.5. I need to calculate the Mean-free-path (MFP) for the...

18 August 2020 9,804 3 View

Is ACE inhibition linked to COVID-19?

There is increasing chatter that proteins in the ACE2 receptor binding domain are related to COVID-19 entering humans cells and subsequently infecting the host. Is there any evidence of ACE...

19 March 2020 6,635 8 View

In Phospharic Acid plant , Calendria is being used is generally of Graphite tube- shell & tube heat exchanger. Anyone using experience SS316 tubes?

Presently Majority of PA plants are using Graphite tubes in their system oh concentration for heat-exchange. Reliability is little less. We want to know that is ANY PA producing plants is having...

13 March 2020 4,877 3 View

How to model blind bolts in ABAQUS?

I am trying to simulate a beam-column connection using blind bolts. but I am not getting an idea to model blind bolts in Abaqus. please give me some idea of modelling. thanks in advance

05 February 2020 8,916 3 View

What are the possible ways to increase the internodal distance of in vitro cultures?

I am working on Papaya micropropagation where I am getting plenty of shoots but they are not increasing in height. I have tried diffuse light, GA3 but did not see improvement. Please suggest a...

03 June 2019 812 23 View

Reduction in fasting Insulin levels and associated CVD/T2D risk?

Does anyone have papers relating fasting insulin levels and associated CVD risks?

03 November 2018 4,281 3 View

What is the relationship between Stroke Volume and Diastolic BP?

Does stroke volume and diastolic BP have a positive linear correlation? Given greater pre-load and thus end-diastolic volume is it expected that diastolic BP would be reduced?

14 December 2017 9,316 4 View

Is RM-ANOVA robust enough with a non-normal dataset?

Does anyone have papers saying that repeated-measures ANOVA has sufficient robustness when analysing a non-normal dataset?

30 November 2017 7,862 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View