Tell me about it!!! Regrettably, we spent $1000 on a bottle of Exoquick, tried it a few times, got tons of contaminants (vs ultracentrifugation), and then found the patent while searching for an explanation. The amount of PEG in a $1000 bottle of Exoquick costs ~$50 from Sigma.
This reagent and System Bioscience's "Exoquick" are basically the same thing - a polyethylene glycol solution. They precipitate everything in your sample - vesicles AND proteins/ contaminants.
Here is the link for the Exoquick patent:
http://www.google.com/patents/WO2013188832A1?cl=en
Tell me about it!!! Regrettably, we spent $1000 on a bottle of Exoquick, tried it a few times, got tons of contaminants (vs ultracentrifugation), and then found the patent while searching for an explanation. The amount of PEG in a $1000 bottle of Exoquick costs ~$50 from Sigma.
PS- I also recommend the ISEV "Position Papers" which discuss exosomes methods and applications. This one is a must read.
http://www.ncbi.nlm.nih.gov/pubmed/24009894
J Extracell Vesicles. 2013 May 27;2. doi: 10.3402/jev.v2i0.20360. eCollection 2013.
Standardization of sample collection, isolation and analysis methods in extracellular vesicle research.
We developed a method to analyze vesicle subsets by IF. Over the course of dozens of experiments, markers such as CD63, CD9, and LAMP1 labeled approx 40-60% of the exosomes. Some of that data is included in the paper below:
Bacterial Membrane Vesicles Mediate the Release of Mycobacterium tuberculosis Lipoglycans and Lipoproteins from Infected Macrophages.
Athman JJ, Wang Y, McDonald DJ, Boom WH, Harding CV, Wearsch PA.
J Immunol. 2015 Aug 1;195(3):1044-53. doi: 10.4049/jimmunol.1402894. Epub 2015 Jun 24.
FYI: JSR micro sells an exosome bead capture kit based on 4 markers (ExoCap)
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