Carl Grim

5 Questions 9 Answers 0 Followers

Questions related from Carl Grim

Any advice for generating a single cells suspension of Tongue epithelium?

Hi all, I am trying to take a tongue epithelium and generate single cells from it for flow. So far, I have had no luck. Getting the epithelium separated from the underlying tissue is easy enough...

30 September 2019 6,257 1 View

Is There a Solid Method for Isolating MSCs from Human Colon Samples?

Hi. I wish to start attempting to isolate and experiment with Mesenchymal Stem Cells from human colon samples. However, it seems that all the procedures I come across are essentially the same as...

11 December 2017 202 7 View

What are your methods for determining total eluted volume of water for mRNA extraction?

Hello, all. In my current lab, when we have completed an mRNA extraction, we record the total volume eluted during the final spin, then use that along with determined concentration to equalize all...

23 July 2017 3,791 2 View

What is the best/most used protocol are used for chondrogenic differentiation of Mesenchymal Stem Cells?

Right now I have a protocol from Lifeline Cell Tech. that requires one to make chondrogenic microbeads from MSCs using an alginate solution and their proprietary media. However, I have seen...

25 August 2016 5,560 5 View

Anyone have any experience with ThermoFisher Total Exosome Isolation Reagent (from cell culture media) Catalog # 4478359?

I am wondering if to go with that kit, or just tag with CD63 and isolate.

27 January 2016 1,338 9 View