Help with multivariate test selection?

Hi all, I have a dataset containing 2 independent variables (1. Region [North, East, South and West] and 2. Year [2010, 2015, and 2020]) and 4 continuous dependent variables (e.g. height, etc.)....

26 January 2021 9,329 3 View

How to compare individual spectra of a scan to a mean of the whole scan?

I'm doing a project, where I scan over a sample recording a spectrum at each position. Although the sample is supposed uniform, there's a large difference in the overall spectral response of each...

13 July 2020 2,077 3 View

What is your experience on practicioning as opposed to doing academic research?

Lately, I have been thinking much about the relationship between working practical tasks, on the one hand, and doing scientific research, on the other. What is your experience..? Most...

01 August 2019 4,989 4 View

How to prepare a freshwater sample for chlorophyll-a measruement using a microplate reader?

I would like to use a microplate reader for chlorphyll-a measurements in freshwtaer samples. I found a lot on the measruemnt itself, but I am still not sure about my sample preperation and...

14 May 2019 1,831 2 View

Using Material Studio I understand that you can simulate polymer blends. Is polymers proportion included?

Looking at the Internet available Material Studio tutorials for polymer blends, I understand that you can simulate the blends but the procedure does not include the polymers proportions amongst...

13 May 2019 4,449 3 View

How do I measure the major axis (longest axis) of an object in a nifti segmentation file?

Hi! I need to measure the maximum length of a random shaped object in a 3D binary segmented nifti volume. Is there any toolbox or any easy approach in Matlab or Python? Thanks in advance! Thorsten

05 July 2018 2,754 1 View

Under which conditions are Mie Scattering Amplitudes imaginary?

Hi, I am using Maetzlers Mie Scattering Script from 2002 to calculate Phase Matrix entries. But under no conditions I am able to produce imaginary scattering amplitudes S1 and S2 which is kind of...

11 November 2015 3,806 4 View

What is the correct terminology for the following two learning phenomena?

What is the phenomena called when a student or pupil can learn better because he or she can associate the new knowledge with something previously known? And: What is the phenomena called when a...

05 February 2014 3,043 8 View

Metric Modeling and Representation of 4D Data through 3D Vector Tensoring

01 January 1970 691 2 View

How to stain membranes independent of membrane proteins?

Dear all, is there any stain or technique to visualize membranes (e.g. nuclear membrane) without using an antibody for membranous proteins? E.g. any dye that incorporates in all the different...

01 January 1970 195 2 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

I am looking for an antibody to Arc protein for use in immunohistochemistry with rhesus monkey brain tissue?

Looking for a well-validated antibody to detect Arc protein expression in paraformaldehyde fixed brain tissue using immunohistochemistry. Ideally this antibody would have been published on using...

07 February 2021 8,457 1 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

Antibody dilution for IHQ in OCT frozen tissue is more similar to IHC in Paraffin-embedded samples or to Immunocytochemistry?

31 January 2021 8,678 3 View

Any tips on Fab blocking of mouse tissue?

Hi, everyone, I am working on immunostaining of bone marrow and growth plates of mice. And I have to use an mouse monoclonal Ab to do this staining. However, with only secondary goat anti-mouse...

21 January 2021 9,826 3 View

Fluorojade-C troubleshooting experience?

Hello all, I've tried a Fluorojade-C stain according to the manufacturer's recommendations on FFPE tissue sections. My problem is that there is no staining, not even the DAPI control. I prepared...

11 January 2021 9,749 0 View

Antigen retrieval for neural cell marker on frozen brain tissue?

Hi, I'm having problems with the Recombinant Anti-MAP2 antibody from Abcam (Recombinant Anti-MAP2 antibody [EPR19691] (ab183830)) antibody on my frozen, 4%PFA perfused, mice brain tissue. I...

10 January 2021 4,002 5 View

How to approach quantification of cytoplasmic immunofluorescent signal in adipocytes?

Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...

05 January 2021 5,420 1 View