How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Trouble shooting with his tagged protein expression in E.Coli?

i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions

15 February 2021 8,939 3 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

12 January 2021 3,606 2 View

Can light waves induce electricity through induction?

Can light waves induce electricity? I am unclear on this matter. Thank you.

30 December 2020 7,974 3 View

What is volume of gas sample to be injected into GC for analysis?

I'm currently working on a batch type gas phase photocatalytic reaction. For product analysis, i draw the gas samples from the reactor using a gas tight syringe and then inject directly into the...

22 December 2020 6,850 2 View

Why the western blot of HIF1alpha of the whole cell lysate shows intense band under normoxia without any induction of hypoxia?

I am working with oral cancer cell lines and trying to find the expressional changes of HIF1alpha under different experimental treatment. I am getting intense band of HIF1alpha in the western blot...

29 November 2020 5,707 3 View

What is the relationship between induction machine stator reactance variation and slip?

It is possible to notice, at some three phase induction machine datasheets, that exists a relationship between stator leakeage reactance and slip, that is, the stator leakage reactance increases...

23 November 2020 2,949 3 View

How to identify GC peaks for FAME mix which do not match reference peaks?

Hello, I am analyzing 37 component FAME mix using GC. I have got 37 peaks and 25 of them show expected order and height. However, others are not in the similar order with the reference for same...

22 November 2020 7,033 4 View

What is the best column temperature program to analyze BTEX concentration in dichloromethane extractant using GC-FID method with hydrogen carrier gas?

I tried the temperature program below to draw my calibration curve using splitless injection but the peaks I got were so small and broad in the concentration range of 5 to 50 ppm. I was wondering...

19 November 2020 4,531 3 View

In online GC-FID/TCD connect to flow reactor, why I am getting instable peak area in FID in every injection under same conditions?

We have online GC-FID and GC-TCD (Mod: 2014) connected with flow reactor system. This GC-FID/TCD is connected to a gas line coming from flow reactor system which I used for cascade reaction. In...

11 November 2020 4,999 6 View

Can induction air heaters be an alternative for HVAC applications in buildings or Electric Vehicles?

Induction air heaters are first introduced as hygienic heaters. These days, while the world is influenced by the Pandemic, can induction air heaters be an alternative instead of all other heaters,...

26 October 2020 8,258 4 View