How to compare individual spectra of a scan to a mean of the whole scan?

I'm doing a project, where I scan over a sample recording a spectrum at each position. Although the sample is supposed uniform, there's a large difference in the overall spectral response of each...

13 July 2020 2,077 3 View

What is your experience on practicioning as opposed to doing academic research?

Lately, I have been thinking much about the relationship between working practical tasks, on the one hand, and doing scientific research, on the other. What is your experience..? Most...

01 August 2019 4,989 4 View

How to prepare a freshwater sample for chlorophyll-a measruement using a microplate reader?

I would like to use a microplate reader for chlorphyll-a measurements in freshwtaer samples. I found a lot on the measruemnt itself, but I am still not sure about my sample preperation and...

14 May 2019 1,831 2 View

How do I measure the major axis (longest axis) of an object in a nifti segmentation file?

Hi! I need to measure the maximum length of a random shaped object in a 3D binary segmented nifti volume. Is there any toolbox or any easy approach in Matlab or Python? Thanks in advance! Thorsten

05 July 2018 2,754 1 View

Under which conditions are Mie Scattering Amplitudes imaginary?

Hi, I am using Maetzlers Mie Scattering Script from 2002 to calculate Phase Matrix entries. But under no conditions I am able to produce imaginary scattering amplitudes S1 and S2 which is kind of...

11 November 2015 3,806 4 View

Anyone familiar with VHL immunohistochemistry on murine sections?

We are looking for an antibody that can detect murine VHL (von Hippel-Lindau) via immunohistochemistry, preferably on FFPE sections. Many thanks and best wishes! Thorsten

03 September 2015 9,737 3 View

What is the correct terminology for the following two learning phenomena?

What is the phenomena called when a student or pupil can learn better because he or she can associate the new knowledge with something previously known? And: What is the phenomena called when a...

05 February 2014 3,043 8 View

Metric Modeling and Representation of 4D Data through 3D Vector Tensoring

01 January 1970 691 2 View

How to stain membranes independent of membrane proteins?

Dear all, is there any stain or technique to visualize membranes (e.g. nuclear membrane) without using an antibody for membranous proteins? E.g. any dye that incorporates in all the different...

01 January 1970 195 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View