I would like to use it, but is totally unfamiliar with it. I have been using Biolog Ecoplates, but the inventor of the plates suggested I tried Microresp.
I need to assess the soil fungal community. Any ideas? Rose Bengal plates is a possibility as is glomalin production. Anything else? I haven't done any work on fungi yet.
01 February 2020 6,034 4 View
I am looking at urease, glycosidase and phosphatase (acid) at this stage. The protocols I have all involve hordes of flasks. Is there shorter methods available? Shorter in the sense of using...
07 August 2019 6,952 4 View
Anyone using Solvita for either nitrogen or carbon measurement? I have started using it and then they changed the protocol and it seems the new protocol doesn't work as well as the old one for my...
06 July 2018 1,557 0 View
I have claye soils that I want to use Biolog Ecoplates on. Can I use CaCl2 (1ml of 1M in 100 ml of 10% soil solution) to settle the soil? Or maybe centrifuge (5000 rpm for 1 min)? I do not want...
10 November 2017 6,269 2 View
10 November 2015 6,933 2 View
I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...
02 March 2021 1,408 1 View
We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...
02 March 2021 3,713 2 View
Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...
02 March 2021 3,584 1 View
We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...
28 February 2021 1,149 1 View
Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...
28 February 2021 7,292 3 View
Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...
26 February 2021 6,151 2 View
I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...
25 February 2021 3,221 7 View
Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...
24 February 2021 5,072 1 View
24 February 2021 1,365 3 View
I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...
23 February 2021 9,933 1 View