I am looking at urease, glycosidase and phosphatase (acid) at this stage. The protocols I have all involve hordes of flasks. Is there shorter methods available? Shorter in the sense of using less chemicals and using microtiter plates.
Are you simply determining the presence of these enzymatic activities in soil sample or you need to isolate microbes producing such activities?
Dear Anelia,
Prof SHamsher is right; Could you clarify more. Unless your soil is chemically contaminated, it may not degrade the mentioned substrates. If you are attempting to analyze the microbial community, which may produce these enzymes, I would recommend you to head to a serially diluted plating of the microbial load, and screen them using colorimetirc (plate assays using the substrate), which would allow the formation of zones of clearance around the substrates.
I just need to determine the presence of the enzymes in agricultural soil. But my question is, is there a microtiter plate method? The methods I have is very cumbersome.
I will appreciate if you be more specific about the enzyme(s) you want to assay in the soil sample. You can prepare an extract of a weighed (say 10 g soil/ 100 ml buffer) and assay the desired enzyme(s) in the soil suspension.
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