How to recrystallization amine compound and it is not soluble in common organic solvents ?

How to recrystallization amine compound and it is not soluble in common organic solvents ?

19 January 2021 671 3 View

What is the ACTUAL COST OF A PUBLICATION??

Hi. It's 2021 January. Recently, I got to know that Elsevier has converted around 160 journals into some "Transformative Journals" and follow the recommendations under "Plan S"...

19 January 2021 9,985 2 View

What if an author has concluded wrong facts in his article and published in Elsevier ?

The question is that in what form of publication we can show true facts and prove the wrong facts invalid ?

29 December 2020 9,727 7 View

What is the difference between filler and fiber?

Explain in correct manner

28 December 2020 5,199 2 View

What should be done if someone 100% copied our article and published in a predatory journal ?

28 December 2020 8,982 10 View

Advantages and Disadvantages of Submitting Article in Special Issues of a Journal ?

What are those ?

21 December 2020 7,757 4 View

What is U matrix for Hosking & Wallis discordancy test based on L-comoments?

Hello everyone I would compute Hosking & Wallis discordancy test based on L-comoments for multivariate Regional Frequency Analysis. Please help me by answer to these questions. 1- Is that...

28 November 2020 8,152 2 View

In microscopy why "smaller the aperture, greater the depth of field"?

12 October 2020 3,636 2 View

How is the resolution of an image affected by the NA of the lens in microscopy?

NA= Numerical Aperture

12 October 2020 2,514 2 View

Which will have a larger Depth of Field: A Light Microscope or an Electron Microscope? Why/How?

The depth of field is the range of distance along the optical axis in which the specimen can move without detectable loss of sharpness.

11 October 2020 4,623 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View