Mohit Kwatra

8 Questions 10 Answers 0 Followers

Questions related from Mohit Kwatra

SHSY-5Y cells (Human Neuroblastoma cells) ATCC -microbial contamination problem is arising, please help me to share the trouble shoot for resolve it.?

Dear Researchers/Experts, I have been trying to maintain the SHSY-5Y cells (purchased from ATCC) in DMEM-F12 (1:1) + 10% FBS (Gibco invitrogen-1shot FBS)+ 1% Antibiotic-Antimyotic (Gibco...

24 October 2018 1,772 4 View

SIte specific Adenovirus targeting?

The paper below knocks out both allele of B2M gene using adenovirus. https://www.sciencedirect.com/science/article/pii/S152500161632617X They have targeted B2M allele by transducting adenovirus...

21 August 2018 5,023 1 View

How do I measure mitochondrial complexes activities in mouse brain?

Dear Researchers, I am working on depression model of mouse brain. I wish to perform the mitochondrial complexes activity assay in mouse brain different parts (striatum, hippocampus, substantia...

09 January 2016 7,951 3 View

Any advice on nuclear, cytoplasmic and mitochondrial extract preparation and proteins extract from the portion of cells?

Dear Researchers, I have to use the nuclear, cytoplasmic and mitochondrial extract for my studies involving m-RNA and protein in brain isolated parts (striatum, substantia nigra, hippocampus,...

24 December 2015 4,870 2 View

Neurotransmitters in Brain (striatum, hippocampus, cortex) measurement through HPLC?

I am working on animal model of motor disorders. I have to estimate the various monoamines (5-HT, NE, DA) in striatum, hippocampus, cortex region of brain. I wish to work on HPLC-electrochemical...

30 November 2015 9,439 5 View

Does anybody know about animal tracking video software for neurobehavioural studies (Morris maze test, NORT, open field etc)?

I am working on Neurobehavioural Projects of rodents as animal model for Neurological disorders related to cognition deficits, depressive, anxiety-depressive behavior. I have a Tech-com (webcam)...

19 November 2015 9,285 5 View

Anyone familiar with Microglial cell line preparation?

Can anybody tell me if anybody having microglial cell line (murine or human)? How can I get it ? If it would be difficult to obtain then how can I isolate (with specific procedure) from rat/mouse...

23 August 2015 2,444 3 View

Can someone provide the ELISA protocol for Rat IL-6 and IL-10 from the Sigma kits?

I lost the protocol of 2 Rat ELISA kits of sigma (IL-10; RAB0246-1KT;...

06 June 2015 2,962 3 View