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Questions related from Yuan Li
Hi, I am working on a project to analyze protein interactions from PBMC. Our problem is that PBMC cannot be extracted from human blood samples immediately, so we just centrifuge the samples to...
22 May 2019 8,798 4 View
I want to explore the effect of different stimuli on brian glucose metabolism in mice. Now I have series of PET/CT images from microPET/CT scan. I can see the differences from these images but I...
23 January 2019 7,390 3 View
Recently, I have been used the RED system to delete the target gene in EPEC. According to the paper, the primer uesd to amplify the selectable marker (cat) is flanked by 50 bp of tir...
19 August 2018 8,994 1 View
Hexokinase I is predominant form of hexokinase family in mammalian brain. But under hypoxic conditions, there is no obvious change in its expression. In contrast, hexokinase II, which is mainly...
19 May 2015 9,789 4 View
When I expose my primary cortical neurons to hypoxia(1%) in a professional hypoxic workstation, only about 10% of the cells die at 24 hrs. Even at 48 hrs, it just reaches up to 20%-30%. Is it...
03 April 2015 3,161 6 View
1) As we all known, in clinical trials,the NMDAR antagonists show prominent neuroprotective effect. However, all of them failed in clinical trials. 2) Why should t-PA should be used within...
31 July 2014 3,775 5 View
Because of the high yield of microglia from the mixed culture , I tried to cryopreserve my purified microglia, and after anabiosis, cellular morphology changed along with time. So has somebody...
30 October 2013 2,767 42 View
In order to test the effect of a molecule (A) on metabolic patterns, I analyzed changes of ECAR and OCR in neurons and astrocutes. In neurons, A augmented ECAR but had no effect on OCR. However,...
01 January 1970 1,800 2 View
