Hi all,
Are there macrophage depletion strategies that work without the use of clodronate or other bisphosphonates?
CD11b-DTR transgenic mice where CD11b+ cell depletion can be done with diphtheria toxin (around (20 ng/g body weight, Sigma-Aldrich IP).
Hi Shalin. Anti-M-CSF-R. Roche have an antibody that kills most resident macrophages. However, since you often study precursors and their development it will most certainly affect myelopoiesis. But it may not be worse than the CD11b-DTR proposition which will kill cDC2s, neutrophils, monocytes, eosinophils, some NK cells, etc. We have a mouse model that allows killing Kupffer Cell specifically but if you would like to kill all macrophages at once it will not be simple... Good luck!
Unfortunately, Clodronate Liposomes is the best method to deplete macrophages depending on the route of administration and what population/location of macrophages is the aim for depletion.
Martin, This csf1-R antibody sounds like an interesting alternative. Do you have any more info? Shalin, is it a specific population or all macrophages? As you could cross some of these specific cre mce with the iDTR f/f mice.
Everyone, thanks for your feedback. I'm looking for a chemical/biologic not genetic approach, and that could be used in humans. Specifically want to deplete the perivascular skin macrophages.
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