Fane Mensah

3 Questions 3 Answers 0 Followers

Questions related from Fane Mensah

Any suggestions on how to measure autophagy in cultured cells? I have used Cyto-ID, but I would like to use another technique in addition.

Autophagy-related proteins that could be stained or measured?

06 June 2017 2,567 2 View

Hi is there anyone who has experience with cell lines for human B cells, if so could you let me know which one and the desired purpose of it?

I would like to optimise some newly developed techniques to study human B cell maturation and differentiation. The only problem is quit a lot of blood is needed to isolate B cells from PBMCs. I...

06 September 2016 6,078 2 View

Could anyone advise us on how we can detect Glucocorticoid receptor beta in lymphocytes (Bcells/Tcells/NK Cells), using any technique (flow, PCR)?

WE have tried PCR (using Lightcycler) but could not detect GCR beta We are now trying to find another technique, looking at flow for example. It is known that beta is around 1000x lower in...

24 March 2016 4,574 1 View