How do you interpret a negative effect for a significant indirect effect in mediation?

X - M is significant positive M - Y is significant negative I understand each of these regressions however, my indirect effect is significant negative? what does that mean?

29 February 2020 8,365 3 View

How do i find out if marital problems and overall happiness correlate to alcohol consumption?

I need to use SPSS to see if the first 2 predictor variables correlate and by how much to OV of alcohol consumption. Multiple regression? How do i input this data into SPSS, whats tests do i run...

02 March 2019 6,129 5 View

IDT vs Sigma?

I work in a big lab that is considering switching from IDT to Sigma to save on shipping costs. Has anybody used Sigma for ordering your oligos? What do you think about the quality of their...

09 March 2017 3,250 3 View

Why are my spinal cord sections curled after IHCs?

25 February 2015 8,011 5 View

Has anyone used PolyJet Transfection reagent with Stealth RNAi?

All the protocols I've found are for DNA plasmids but I don't know if that means it definitely wouldn't work for RNAi.

04 October 2012 7,929 2 View

Schizotypal & rejection sensitivity/social alienation

I've recently been thinking about how rejection sensitivity is associated with social alienation (partly due to self-sabotaging behaviors/the self-fulfilling prophecy) among individuals with...

01 January 1970 2,313 5 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

I am looking for an antibody to Arc protein for use in immunohistochemistry with rhesus monkey brain tissue?

Looking for a well-validated antibody to detect Arc protein expression in paraformaldehyde fixed brain tissue using immunohistochemistry. Ideally this antibody would have been published on using...

07 February 2021 8,457 1 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

Antibody dilution for IHQ in OCT frozen tissue is more similar to IHC in Paraffin-embedded samples or to Immunocytochemistry?

31 January 2021 8,678 3 View

Any tips on Fab blocking of mouse tissue?

Hi, everyone, I am working on immunostaining of bone marrow and growth plates of mice. And I have to use an mouse monoclonal Ab to do this staining. However, with only secondary goat anti-mouse...

21 January 2021 9,826 3 View

Antigen retrieval for neural cell marker on frozen brain tissue?

Hi, I'm having problems with the Recombinant Anti-MAP2 antibody from Abcam (Recombinant Anti-MAP2 antibody [EPR19691] (ab183830)) antibody on my frozen, 4%PFA perfused, mice brain tissue. I...

10 January 2021 4,002 5 View

How to approach quantification of cytoplasmic immunofluorescent signal in adipocytes?

Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...

05 January 2021 5,420 1 View

Can you recommend a protocol for immunohistochemistry of mouse brain sections using anti-MAP2?

Current protocol does not give clear images.

04 January 2021 1,614 3 View

I need to know about immunohistochemistry steps?

Immunohistochemistry staining. Tissue Fixation during IHC. Using DAB. blocking step. controls group.

23 December 2020 5,768 2 View

ISO antibody against BFP for FFPE IHC?

Suggestions and recommendations welcome. Any usage/protocol experience would also be helpful/appreciated. Cheers

22 December 2020 599 3 View