What can lead to DNA damage of spermatozoa during epididymal transit in the absence of ROS positive semen samples?
Hi Stefen. What is the type of semen i.e animal or human semen? Did you take the semen sample directly from epididymus or after ejaculation? Did you assess the enzymes activity, heavy metal eliments like cadimium or arsinic...ect.?
You may have a semen that has average ROS levels; this does not necessarily mean that during epididymal transit spermatozoa were not exposed to ROS. Seminal vesicles and prostate fluids may buffer completely ROS coming from the epididymal fluid.
Thanks for all the suggestions. Here is some more information:
1) Healthy donors with no varicocele
2) Human samples and semen obtained after ejaculation
What if the origin is in the seminiferous tubules (testicular source), some days before the sparmatozoa reach the head or the tail of the spermatozoa?
I.e. Source: heat stress, vaccination, etc. --> 25-35 days before ejaculation. Probably, the epididymis won't be affected 15-20 days after (10 days before ejaculation), will it?
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