What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to optimize reaction conditions to achieve maximum lanthanide chelate attachment of the surface a polymer encapsulated nanoparticle?

Hi, The aim of this project is to attach a lanthanide chelate onto the surface of a polymeric nanoparticle that is encapsulated with polystyrene maleic anhydride (the anhydride hydrolysis in...

29 September 2020 7,386 2 View

List of ISI/Scopus indexed journal with no processing fee. In the area of medical education/public health/community medicine/health science.?

Hii expert, I am a first-year PG student enrolled in Public Health (Community Medicine). I am looking for a list of Scopus/ISI indexed journals with no processing fee. In the area of medical...

14 July 2020 4,496 2 View

How to fix exosomes prior to labeling and confocal?

Can anyone suggest a protocol (with reference) on how to prepare the exosomes for CONFOCAL STAINING? Details- exosmes will be isolated from culture media at 100,000g and will be resuspended in...

30 June 2020 6,327 2 View

How to combine received signals to a cross polarized antenna in a mobile?

Does anybody know if we have a cross polarized antenna in a smartphone how the receive signal in the two ports usually combines? is using a 90 degree hybrid coupler a normal way to combine the...

10 October 2019 9,848 1 View

How can I fix my western blot membrane ?

I'm a beginner at western blotting, and I have hight background, However I don't know whether it comes from the primary or the secondary antibody. and I thought I could start over and incubate...

03 October 2019 5,297 4 View

What is the right method to identify microbes from commercial effective microorganisms?

First, how to isolate DNA from effective microorganisms? is it the more to genomic DNA isolation or metagenomic DNA isolation protocol? Second, is the Bacterial 16s rRNA Amplicon Sequencing with...

23 September 2019 8,440 8 View

Do you know any paper that used cross correlation coefficient calculation to find similarity between two wide-band channels ?

Hi everyone, I calculated cross correlation coefficient to see how much two received signals to the ports of a cross polarized antenna are similar, I'm looking for a paper (publication) that used...

05 September 2019 1,091 3 View

Dear colleagues, are there any free online English editing services?

Are there any free online English editing services?

10 April 2019 1,424 3 View

How can I calculate the cortical thickness and different brain volumes of segmented MRI images ?

I have 3T, T1- weighted, MR images of marmoset brain. I have segmented these images into GM, WM and CSF (using SPM). My aim is to calculate the cortical thickness and the brain compartment's...

19 March 2019 916 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View