Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

What is the best way to improve the working potential window of a solid electrolyte?

My solid polymer electrolyte shows low bulk resistance show quite a low bulk resistance. However, the potential window (obtained from LVS) is very small. Please how can I improve the operating...

31 January 2021 3,340 9 View

What is the best procedure for measuring the pH of aqueous colloidal systems?

There has long been a debate among my colleagues about measuring the pH of aqueous colloids. As I see there is no general truth in the matter, so I ask this question below. What is the best and...

31 January 2021 8,203 3 View

Is carboxymethyl cellulose a better electrolyte host polymer than methyl cellulose and cellulose acetate?

I want to prepare a cellulose-based biopolymer solid electrolyte by solution casting technique. However, choosing the right cellulose derivative has been my major challenge. Among the many factors...

30 January 2021 5,355 3 View

How can I implement a Generalized Estimating Equation method on spatially autocorrelated time-series data?

I used Generalized Estimating Equations (geepack in R) on my regularly monitored (twice a year) plant abundance data in grid cells to evaluate their trends of density. Since these are time-series...

26 January 2021 3,833 1 View

How to extract isodose lines from radiotherapy dose cloud into masks that can be overlaid on images using python?

I'm able to load a dose cloud into python as a numpy ndarray using pynrrd. The next challenge is to extract isodose lines (like V50%) to create masks (bitmap images) that I can overlay onto other...

19 January 2021 8,503 2 View

How to calculate percent of identity in aligned RNA sequences with a sliding window ?

Hello, I aligned some RNA sequences to identify if some part are similar. I would like to represent my result with the similarity in y-axis and the alignement in x-axis. I want to calculate this...

13 January 2021 7,833 3 View

Which peak should I consider when calculating ZIF-8's crystalline size from XRD?

Hello, Using the Scherrer's equation, one could calculate, roughly, the crystalline size of ZIF-8. However, I am confused as to which peak in the XRD pattern should be taken for the calculation?...

10 January 2021 8,633 3 View

Bio Rad CFX96 Touch Real time PCR System?

Dear friends I would like to ask about your experience with the instrument (Bio Rad CFX96 Touch Real time PCR System) and its performance. Also I would like to know if this version need...

29 November 2020 2,166 5 View

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02 March 2021 3,060 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View