First of all, you have to activate the pure strains of that fungi in PDB, then make mixture of PDB and your feed media step by step, to adopt the fungi on your natural media, after that you have the mother culture that you can use it as a bulk culture later, but all of these steps must be in sterilised media. Such suggestions from my opinion about the same conditions.   

In fact, I know the steps of how to inoculate feed with fungus, what I'm asking is how to do that in large scale? For example, I need to incubate 100kg of feed with specific strain, which won't be done in laboratory. So how to do that?

Dear Khalil

there are vats specified for that you can make yourself like cheese vats with double jacket in order to keep the temperature of your mix in the same time these vats made from stateless so the feed will be safe from metal contamination. in order to do your work it so easy to sterilize the vats, the feed mix the continua your experimental

shenana 

Dear Prof Shenana,

Thanks for your informative reply.

Mr. Khalil, first make one point clear on your purpose: inoculating a fungus to a large volume of feed, what is your next step: to incubate it for  further culture/ fermentation, or just to use it as a premix for feed? In any case, the best way to ensure homogenous mixing, it is better to mix (inoculate) fungal spores rather than its vegetative mycelia. For better mixing you may use a large scale blender used for mixing different types of powdery food or feed ingredients (for example, instant soup formulas or feed premix) or you can use a more simple device like a concrete mixer (often seen in construction sites), if you don't need a strict sanitation. It depends on your next step. Good luck.

Hi Khalil

Some references 1

https://books.google.iq/books?id=Qeg4DwAAQBAJ&pg=PA401&lpg=PA401&dq=inoculate+large+quantity+of+feed+with+fungus&source=bl&ots=6K1mZpMooj&sig=aJpLeMo2uv5-Bb-MUORnU6tOkjQ&hl=en&sa=X&ved=0ahUKEwi9iamLjsfXAhWE0RoKHU3aCccQ6AEILjAC

2

https://books.google.iq/books?id=58c-HPij6RMC&pg=PA123&lpg=PA123&dq=inoculate+large+quantity+of+feed+with+fungus&source=bl&ots=yZ5urEqv3Z&sig=LXIAksUBEmIY9ToiAKxfsqWcf3g&hl=en&sa=X&ved=0ahUKEwi9iamLjsfXAhWE0RoKHU3aCccQ6AEIMzAD

3

https://books.google.iq/books?id=NdLx93G2SX4C&pg=PA265&lpg=PA265&dq=inoculate+large+quantity+of+feed+with+fungus&source=bl&ots=mBOp6-ij3r&sig=vJmXCgv-ICLUZFGNZJLGUkpVpWw&hl=en&sa=X&ved=0ahUKEwi9iamLjsfXAhWE0RoKHU3aCccQ6AEINTAE

4

https://books.google.iq/books?id=Vr75NtPym-YC&pg=PA104&lpg=PA104&dq=inoculate+large+quantity+of+feed+with+fungus&source=bl&ots=d0v1g0Wz6E&sig=RlcGkgFnWCEi18i1phjW0XrZAlY&hl=en&sa=X&ved=0ahUKEwi9iamLjsfXAhWE0RoKHU3aCccQ6AEIODAF

5

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=7&cad=rja&uact=8&ved=0ahUKEwi9iamLjsfXAhWE0RoKHU3aCccQFgg9MAY&url=http%3A%2F%2Fwww.pnas.org%2Fcontent%2F100%2F26%2F15643.full&usg=AOvVaw2mXqULLdiU_pBtUdfWXxcR

6

https://books.google.iq/books?id=Jk0Hym1yF0cC&pg=PA195&lpg=PA195&dq=inoculate+large+quantity+of+feed+with+fungus&source=bl&ots=TyZKn1A1xN&sig=JdngtUqYg9FoUTVL6EtA9ARMmV4&hl=en&sa=X&ved=0ahUKEwi9iamLjsfXAhWE0RoKHU3aCccQ6AEIRjAH

7

https://books.google.iq/books?id=-FrqtgJ4cfMC&pg=PA939&lpg=PA939&dq=inoculate+large+quantity+of+feed+with+fungus&source=bl&ots=tJ3VCvt35-&sig=r6Ha1Ty0ThhG7r02IO9uKH8Utao&hl=en&sa=X&ved=0ahUKEwi9iamLjsfXAhWE0RoKHU3aCccQ6AEISDAI

7

https://books.google.iq/books?id=-FrqtgJ4cfMC&pg=PA939&lpg=PA939&dq=inoculate+large+quantity+of+feed+with+fungus&source=bl&ots=tJ3VCvt35-&sig=r6Ha1Ty0ThhG7r02IO9uKH8Utao&hl=en&sa=X&ved=0ahUKEwi9iamLjsfXAhWE0RoKHU3aCccQ6AEISDAI

8

https://books.google.iq/books?id=Jk0Hym1yF0cC&pg=PA195&lpg=PA195&dq=inoculate+large+quantity+of+feed+with+fungus&source=bl&ots=TyZKn1A1xN&sig=JdngtUqYg9FoUTVL6EtA9ARMmV4&hl=en&sa=X&ved=0ahUKEwi9iamLjsfXAhWE0RoKHU3aCccQ6AEIRjAH

9

https://books.google.iq/books?id=yd09DwAAQBAJ&pg=PA39&lpg=PA39&dq=inoculate+large+quantity+of+feed+with+fungus&source=bl&ots=6T9CnmsP2M&sig=N3Gfn5VN-0NeJGM3JxUAcNg5NAA&hl=en&sa=X&ved=0ahUKEwi9iamLjsfXAhWE0RoKHU3aCccQ6AEISjAJ

10

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=11&cad=rja&uact=8&ved=0ahUKEwidnrXdlcfXAhVBWhoKHS31Az44ChAWCCUwAA&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC4687392%2F&usg=AOvVaw1ulhP35K3HONRVmBWhEveh

11

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=12&cad=rja&uact=8&ved=0ahUKEwidnrXdlcfXAhVBWhoKHS31Az44ChAWCCswAQ&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC4944425%2F&usg=AOvVaw0bu5Q6SexK7M_uELzJy6kt

12

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=13&cad=rja&uact=8&ved=0ahUKEwidnrXdlcfXAhVBWhoKHS31Az44ChAWCDEwAg&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC4305255%2F&usg=AOvVaw0IbZ0HtTivGvLYniygJShf

13

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=14&cad=rja&uact=8&ved=0ahUKEwidnrXdlcfXAhVBWhoKHS31Az44ChAWCDcwAw&url=https%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2FBF02224884&usg=AOvVaw3vYeoUeE7TH9VQuQTNu4_R

14

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=15&cad=rja&uact=8&ved=0ahUKEwidnrXdlcfXAhVBWhoKHS31Az44ChAWCDwwBA&url=http%3A%2F%2Fonlinelibrary.wiley.com%2Fdoi%2F10.1111%2Fj.1469-8137.2012.04348.x%2Ffull&usg=AOvVaw0VS982i8Fz6vg-y4iSVXgs

15

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=16&cad=rja&uact=8&ved=0ahUKEwidnrXdlcfXAhVBWhoKHS31Az44ChAWCEUwBQ&url=https%3A%2F%2Frodaleinstitute.org%2Fa-complete-how-to-on-farm-am-fungus-inoculum-production%2F&usg=AOvVaw0JL4hw2XpWS_OIZ0u9kqdP

16

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=17&cad=rja&uact=8&ved=0ahUKEwidnrXdlcfXAhVBWhoKHS31Az44ChAWCEwwBg&url=https%3A%2F%2Facademic.oup.com%2Ficb%2Farticle-pdf%2F32%2F4%2F593%2F5974269%2F32-4-593.pdf&usg=AOvVaw0zuZw5TANQOQfse6tFG7pE

17

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=19&cad=rja&uact=8&ved=0ahUKEwidnrXdlcfXAhVBWhoKHS31Az44ChAWCFcwCA&url=http%3A%2F%2Fwww.tandfonline.com%2Fdoi%2Ffull%2F10.1080%2F15572536.2006.11832749&usg=AOvVaw1NA0XSJelZfmptIoLuUXOI

18

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=20&cad=rja&uact=8&ved=0ahUKEwidnrXdlcfXAhVBWhoKHS31Az44ChAWCF0wCQ&url=http%3A%2F%2Fawaytogarden.com%2Ffeed-the-soil-my-experiment-with-mycorrhizae%2F&usg=AOvVaw2dtH1Um2qIeHdnKbuNrltl

19

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=21&cad=rja&uact=8&ved=0ahUKEwjV1oXxmMfXAhWHPBoKHZh6DD84FBAWCCUwAA&url=https%3A%2F%2Fextension.illinois.edu%2Fsoil%2FSoilBiology%2Ffungi.htm&usg=AOvVaw08Xg-XJI00O9tHuoMuD8WK

20

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=26&cad=rja&uact=8&ved=0ahUKEwjV1oXxmMfXAhWHPBoKHZh6DD84FBAWCEQwBQ&url=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep41292&usg=AOvVaw09-XzFTH8LaxkbeF8O3q-M