Dear All,
We tested retrovirus and lentivirus for transduction of murine T cells but had a very poor transduction efficiency (
One way to enhance transduction efficacy could be coating 24-well plates (non-cell culture treated, to enhance coating efficiency) with retronectin and then the anti-CD3 and CD28 antibodies as well as the virus particles and then spin your T cells down for 2.5h at 32°C with 800xG (spin-fection).
Hi Kaifeng,
in my experience, I use FBS to coat in cell culture well, and then suspension cells like K562 cell attach to the surface of a cell culture well, so it causes to increase my transduction rate.
this is my paper link:
Article Improvement of K562 Cell Line Transduction by FBS Mediated A...
Hi Kaifeng,
This might be too late of a response, but in case it still helps somehow. I struggled with the same issue for over a year- turned out, murine CD8+ cells are very resistant to lentivirus. Once we switched to retrovirus, the transduction efficiency significantly improved (usually between 20-50%).
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