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Questions related from Kaifeng Hung
Dear All, We have multiple TCR sequencing data that vary in sequencing depth (with different read counts). May I know how I can overcome the effect of read counts on TCR repertoire...
01 November 2021 5,689 0 View
Hi, I am wondering why our positively-selected CD8 T cells became two population on FSC-SSC plot (as shown below) after activation by using Dynabeads?
13 October 2021 7,449 4 View
Dear All, We tested retrovirus and lentivirus for transduction of murine T cells but had a very poor transduction efficiency (
13 October 2021 2,425 3 View
Dear All, Is there any commercially available TCR-mimic antibody other than anti‑H‑2Kb/SIINFEKL antibody? Either for mouse or human works for us. Thank you very much,
19 August 2020 10,167 2 View
Dear All, Will cryopreservation affect TCR sequence? My plan is to store our PBMC at -80 degree first, and these cells will later be thawed and sorted by FACS for TCR sequencing. Thank you,
19 March 2020 9,921 3 View
I thought that more differentiated cells (like epithelial cells as compared to stem cells) are more resistant to cytotoxicity?
13 November 2017 6,698 1 View
Does PD-1 have to be expressed during the activation of T cells? In other words, is it possible that T cells without the expression of PD-1 are already primed/activated?
01 January 1970 3,714 2 View
Dear All, Are the Tcra-J-alpha26 or Tcrb-D-beta2 and Tcrb-J-beta2.6 segments important to the TCR of OT-1 mouse? If only the J and D segments are replaced by other segments (but Tcra-V2 and...
01 January 1970 5,878 0 View
