Kaifeng Hung

7 Questions 1 Answers 0 Followers

Questions related from Kaifeng Hung

Two population of CD8 T cells after adding Dynabead?

Hi, I am wondering why our positively-selected CD8 T cells became two population on FSC-SSC plot (as shown below) after activation by using Dynabeads?

13 October 2021 7,412 4 View

Any suggestion of murine T cell transduction?

Dear All, We tested retrovirus and lentivirus for transduction of murine T cells but had a very poor transduction efficiency (

13 October 2021 2,404 3 View

Commercially available TCR-mimic antibody?

Dear All, Is there any commercially available TCR-mimic antibody other than anti‑H‑2Kb/SIINFEKL antibody? Either for mouse or human works for us. Thank you very much,

19 August 2020 10,107 2 View

How to store PBMC for TCR sequencing?

Dear All, Will cryopreservation affect TCR sequence? My plan is to store our PBMC at -80 degree first, and these cells will later be thawed and sorted by FACS for TCR sequencing. Thank you,

19 March 2020 9,886 3 View

Why are cancer cells with EMT more resistant to chemotherapy?

I thought that more differentiated cells (like epithelial cells as compared to stem cells) are more resistant to cytotoxicity?

13 November 2017 6,657 1 View

PD-1 and T cell activation

Does PD-1 have to be expressed during the activation of T cells? In other words, is it possible that T cells without the expression of PD-1 are already primed/activated?

01 January 1970 3,683 2 View

How important are the D and J segments to the TCR of OT-1 mice?

Dear All, Are the Tcra-J-alpha26 or Tcrb-D-beta2 and Tcrb-J-beta2.6 segments important to the TCR of OT-1 mouse? If only the J and D segments are replaced by other segments (but Tcra-V2 and...

01 January 1970 5,852 0 View