What is the cause of a smear from direct DNA amplification from human buccal swab samples ?

Dear fellow scientists, I like to performed a direct amplification of a fragment of 222-bp DNA from human genome and use is for further analysis. By using the following methods, I obtained...

07 February 2021 891 3 View

How can I calculate packing density of particles by fomulas or softwares?

I have all data of particles, such as size distribution, or numbers. Is there any way that I can calculate the packing density of these particles? Assume that all particles are circular.

05 January 2021 4,272 2 View

How to measure telomerase activities in TISSUE?

Can anyone recommend a protocol to measure the telomerase activity in tissue samples? Before I was able to measure telomerase activity in cell populations using a telomerase activity measurement...

25 November 2020 1,509 1 View

Why did the heat flow of DSC in the 2nd cycle decrease without any decomposition or volatilizing of sample?

My sample was the copper paste contained PEG, and there were no endothermic peaks or exothermal peaks, so I was wondering why did the heat flow in the 2nd and the 3rd cycle decrease? Thanks!

31 August 2020 7,169 3 View

Commercially available TCR-mimic antibody?

18 August 2020 9,995 2 View

Which software is used to plot graphs in engineering books?

Hello guys, I encounter such this plot (attached) in the engineering books, but don't know which software the authors used. It is not only about the plot itself because plotting observed X-Y...

17 July 2020 8,036 4 View

What resources or articles would you recommend for xenotransplantation procedures?

According to WHO, "the categories of xenotransplantation procedures include the following: Solid organ xenotransplantation; cell and tissue xenotransplantation; extracorporeal perfusion...

03 July 2020 6,962 1 View

Can I use LS column and MidiMACS seperator from Miltenyi Biotec Neutrophil Isolation Kit for mouse peripheral blood?

We are using the neutrophil isolation kit from Miltenyi Biotec to get the neutrophils from peripheral blood from JAK2 constitutively active mice. This mouse is a leukemia model, so the traditional...

11 March 2020 8,611 3 View

Which software tool implemented the algorithm for maximum common edge (connected) subgraph?

I need to find the maximum common edge (connected) subgraph of two input graphs. I have implemented them in Python as follow: I construct line graphs of two input graphs then identify the maximum...

16 December 2019 8,088 1 View

Recommendations for constitutive expression plasmid in K12-BW25113 E.coli?

Dear researchers, I would like to ask for the recommendation for plasmid (kan or chloramphenicol resistance marker) which could constitutively express the E.coli protein (inner membrane protein)...

11 November 2019 7,968 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View