Commercially available TCR-mimic antibody?

More Kaifeng Hung's questions See All

How can I calculate packing density of particles by fomulas or softwares?

I have all data of particles, such as size distribution, or numbers. Is there any way that I can calculate the packing density of these particles? Assume that all particles are circular.

05 January 2021 4,272 2 View

How to measure telomerase activities in TISSUE?

Can anyone recommend a protocol to measure the telomerase activity in tissue samples? Before I was able to measure telomerase activity in cell populations using a telomerase activity measurement...

25 November 2020 1,509 1 View

Why did the heat flow of DSC in the 2nd cycle decrease without any decomposition or volatilizing of sample?

My sample was the copper paste contained PEG, and there were no endothermic peaks or exothermal peaks, so I was wondering why did the heat flow in the 2nd and the 3rd cycle decrease? Thanks!

31 August 2020 7,169 3 View

Which software is used to plot graphs in engineering books?

Hello guys, I encounter such this plot (attached) in the engineering books, but don't know which software the authors used. It is not only about the plot itself because plotting observed X-Y...

17 July 2020 8,036 4 View

DNase specific to plasmid (circular) DNA?

Is there a specific DNase that digest plasmid (circular) DNA and leaving linear blunt-ended DNA intact? For example after doing CRISPR-Cas9 guided digest of a large BAC vector (~100 kb), I want to...

04 March 2020 4,857 3 View

Which software tool implemented the algorithm for maximum common edge (connected) subgraph?

I need to find the maximum common edge (connected) subgraph of two input graphs. I have implemented them in Python as follow: I construct line graphs of two input graphs then identify the maximum...

16 December 2019 8,088 1 View

Lingo problem, how to use the optimization result of the LP model in the calculation section?

Suppose we have a linear programming model in the below: Model: Max = 3*x1+5*x2 x1

02 October 2019 8,061 3 View

How to mesh a tapered plate in ABAQUS CAE?

Hi all, I am new to ABAQUS CAE and is trying to seek help in RG with regard to my research project. I am trying to mesh a marine stabilizing fin (Basically a tapered plate with hinges-solid) and...

11 April 2018 6,753 6 View

Vapor pressure or Antoine coefficients of LiOH and FeSe?

The experiments I'm doing is gas-phase reaction, so that I wonder to know the vapor pressure of LiOH and FeSe. However, I can't find any data or reference on the internet or the handbook that...

02 November 2017 8,323 2 View

Asking about primer design for qPCR?

Hi everyone, I have been facing with a very big challenge to design some primers for qPCR. My problem is that I need to design some primers to test the expression of some stressed genes in...

11 September 2017 7,859 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Russell Linscott

ESK1 ( http://europepmc.org/article/med/26688548) is available from Creative Biolabs: https://www.creativebiolabs.net/Anti-WT1-Recombinant-Antibody-clone-ESK1-23198.htm

Jan-Jaap Verhoef

What I recall no commercial abs are being sold. Most are cross-reactive to other peptide sequences. What you can do is identify sequences from different patents and have those expressed yourself.

Interested to learn if you found any though