Valero Andreu Matillas

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Questions related from Valero Andreu Matillas

Any Substitute Equivalent to Sigma-Aldrich's ExtrAvidin® –Alkaline Phosphatase for ELISpot?

Hello everyone, I am working with ELISpot technique and have been using ExtrAvidin® –Alkaline Phosphatase from Sigma-Aldrich (product code E2636). However, this product seems to be experiencing...

06 June 2023 2,676 1 View

Any help with intracellular IL-17A and IFN-y stainning in stimulated PBMCs?

I am standarizing a protocol to stain Il-17A and IFN-y in PBMCs: I use plates of 24 wells (CAT Nº 142475) from thermoscientific with a cell concentration of 1million/ml. And stimulate cells with...

30 July 2020 5,123 2 View