Does anyone have experience with Transfection with G418 cassette in Leishmania?

08 September 2014 9,579 2 View

What are the protocols for Immunofluorescence in Leishmania?

I check the location of my protein in Leishmania cell. I obtain positive results, the DAPI stain is ok, but I obtain I a high level of non-specific binding, probably due to hydrophobic...

24 February 2014 2,076 5 View

What are the protocols for Immunofluorescence in Leishmania?

I check the location of my protein in Leishmania cell. I obtain positive results, the DAPI stain is ok, but I obtain I a high level of non-specific binding, probably due to hydrophobic...

01 February 2014 3,909 6 View

Protein extract quantification

I am making a total protein extract from a bacterium. In the final buffer I diluted the sample with 7M Urea, 2M Thiourea, 4% NP40, Tris, PMSF, EDTA and DTT. I have tried to quantify the sample...

31 December 2012 9,287 10 View

Which method to use to synchronize validation of emotions in text and video?

I need to compare the emotions of text and video in data previously validated by observers, and that already has a corpus of related emotions.

01 January 1970 9,660 3 View

Which method to use to synchronize validation of emotions in text and video?

I need to compare the emotions of text and video in data previously validated by observers, and that already has a corpus of related emotions.

01 January 1970 4,376 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View