It´s the new version of the old system for express protein in Leishmania tarentolae.
I am trying to do Grounded Theory in documentary research. I have obtained the most frequent terms (induction) to perform deductive coding and so, articulate a hypothesis about the hidden...
05 June 2024 2,599 2 View
Greetings community. Are there any experts in this topic you can recommend to build a solid theoretical revision for a reflection around the topic? Thanks for your help.
10 April 2024 1,592 2 View
I am verifying a methodology for power system reinforcement. I want to test it in Power Factory, but I can't find the IEEE 30 bus system.
02 April 2024 7,073 4 View
Technology has favored communication and interaction among different peoples and individuals throughout history. In the 16th century, the massive arrival of European settlers in the American...
04 November 2023 897 8 View
Can I get the limit of detection just with CT value in qPCR? I dont have concentration neither pfu Thanks
01 October 2023 9,555 1 View
What happen that caused these white spots/build up? Just some background if needed, I cast my own gels and I ran two 15% SDS Page gels today. One of them came out perfect while my second gel...
23 April 2021 8,125 3 View
I am currently contributing to the World Water Day and World Toilet Day campaigns and this data would help.
09 June 2019 6,946 2 View
I'm begining my PhD's degree in Electrical Engineering, for the moment I'm modelling wavelet transform to analize the PMU's information. I want to predict faults in electrical transmission...
16 December 2015 7,720 2 View
Anybody could obtain transfectant cells of Leishmania major Friedlin with the cassette of G418?. I don’t have any problem with others cassette (like Hygromycin and Blasticidin) but with neomycin...
08 September 2014 9,680 2 View
I check the location of my protein in Leishmania cell. I obtain positive results, the DAPI stain is ok, but I obtain I a high level of non-specific binding, probably due to hydrophobic...
24 February 2014 2,182 5 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...
21 July 2024 4,322 1 View