César Poza-Carrión

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Questions related from César Poza-Carrión

Any people work with the new version of constitutive expression vector pLEXSY-hyg2.1?

It´s the new version of the old system for express protein in Leishmania tarentolae.

03 March 2017 7,410 0 View

Does anyone have experience with Transfection with G418 cassette in Leishmania?

Anybody could obtain transfectant cells of Leishmania major Friedlin with the cassette of G418?. I don’t have any problem with others cassette (like Hygromycin and Blasticidin) but with neomycin...

09 September 2014 9,629 2 View

What are the protocols for Immunofluorescence in Leishmania?

I check the location of my protein in Leishmania cell. I obtain positive results, the DAPI stain is ok, but I obtain I a high level of non-specific binding, probably due to hydrophobic...

02 February 2014 3,958 6 View

Protein extract quantification

I am making a total protein extract from a bacterium. In the final buffer I diluted the sample with 7M Urea, 2M Thiourea, 4% NP40, Tris, PMSF, EDTA and DTT. I have tried to quantify the sample...

01 January 2013 9,355 10 View