For Activity staining of SOD, CAT and APX .
sample buffer
250 mM Tris-HCl pH 6.8,
50% (v/v) glycerol,
0.04% (w/v) bromophenol blue)
Carolina Griselda Luchetti
Hello Benedict,
If I understand your question correctly, the tests must be performed with the buffer 1x.
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Haythem Mhadhbi
for activity staining you should use a 50 mM buffer without glycerol or bb
i think you mean loading buffer (sample buffer) to prpare the samples for native electrophoresis. in this case you can use 10x buffer (ex; 10ul of 10x buffer with 90 ùl sample
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