Does anyone have an old digital 'whole slide' scanner that they can donate to a good cause?

New transplant programme starting in a small African nation, but they will need support and training in the examination of biopsies. A digital 'whole slide' scanner would allow virtual biopsy...

27 November 2019 9,900 1 View

Is transgender a feeling or a desire?

I encounter difficulty in getting the sense of transgender. If it is feeling how does it develop? If it is desire what motivates it? It is important that I should have knowledge on this phenomena...

23 July 2019 5,137 4 View

What are possible substitutes for P2O5 as a desiccant?

I've been using phosphorus pentoxide as the desiccant while drying my enzymes; however, our stockroom has now run out of the chemical. What are other chemicals that can be used as desiccants that...

12 January 2018 8,072 4 View

How to calculate the average correlation across samples in a meta analysis?

Dear researchers, I am conducting a meta analysis which analyses the correlations between personal values and different well-being scales across different studies and by that across different...

26 December 2017 748 6 View

Have a PCR product obtained with degenerate primers, need to clone. Interesed in one band specifically, how do I choose the ratio product:vector?

I am using degenerate primers to amplify a family of genes. My results in an agarose gel are 6 bands (862pb, 632pb, 408pb, 335pb and 226pb). The bands of interest are 408pb and 335pb. What...

27 November 2017 7,346 3 View

Do I need longitudinal data for path analysis?

Dear researchers, I plan to investigate possible causalities of certain psychological constructs and variables like Engagement, Goal attainment, Well-being... Based on correlation analyses and...

09 November 2016 3,681 2 View

What's the most recent Portraits Values Questionnaire version (PVQ-RR?)?

I would appreciate it and be very happy if anybody could tell me which is the most recent Portraits Value Questionnaire (PVQ) based on Schwartz et al. 2012 with 57 items (not PVQ21 or PVQ40)?...

20 March 2016 2,614 8 View

Almost all soil analyses carried in the laboratory are done on 2 mm sieved soil. Can someone please explain why?

04 July 2014 4,221 14 View

In a potted maize experiment, what is the appropriate way to collect leachate? What is the appropriate leachate storage temperature and duration?

l am about to carry out a pot experiment and l need to know the appropriate way to collect leachate, its storage temperature and duration and the appropriate leachate sampling interval/period. l...

10 March 2014 9,902 10 View

Docking of trinucleotides to proteins?

Does anyone know of a docking program (preferably a webserver) that can dock small DNA duplexes or di/trinucleotides to proteins?

14 May 2013 5,891 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View