Dengue virus non-permissive cell lines ?????

Do any one have a list of cell lines that are non-permissive to Dengue virus infection?

09 October 2016 1,830 1 View

Global transcriptional repression; Can I spike in RNA as alternative reference control for sybrgreen real time RT-PCR?

02 March 2016 2,784 4 View

How can I use external standard curve to calculate viral copy no.?

03 April 2015 1,636 2 View

How can I group my Mass spec data according to their biological process, molecular function and cellular component using free Bioinformatics software?

Hi, In my experiment I am interested to find interaction partner of the protein of my interest. For that I tagged my Protein with GFP and a pull down experiment was performed and then I analysed...

10 November 2014 1,969 4 View

What are the criteria to choose an appropriate plasmid to construct a stable cell line for expression of an eukaryotic protein?

Stable cell line.

09 October 2013 8,234 7 View

Stability of extracted RNA?

This is a very fundamental and practical problem faced by most of the lab working with molecular biology, mostly with RNA. I would like to discussion in this scientific forum regarding how most of...

08 September 2012 8,348 43 View

GFP-tagged Protein pulldown; not enough to visualize on 10% PAGE

I want to pull down a membrane bound cytoplasmic GFP tagged protein. For this I ordered GFP tagged beads from chromotech. I am lysing the cells expressing GFP tagged protein of my interest with...

08 September 2012 5,250 5 View

Experimental design for Virus and Host Protein Interaction?

I am working with the apoptosis inhibition by RNA virus. I could figure out the protein in my virus which decelerate the apoptosis in its host. I have also learnt from the literature that it uses...

04 May 2012 1,834 7 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View