Would you like to share if you have double GFP or double dsRED plasmid?
Do any one have a list of cell lines that are non-permissive to Dengue virus infection?
09 October 2016 1,929 1 View
I am working on a gene expression experiment using SYBR green RT-PCR. I see perfect single peak melting curves for reference gene in my control samples but curves are erratic for treatment sample...
02 March 2016 2,852 4 View
I am using iq5 bio rad real time pcr machine. I want to use the std curve from my previous experiment to analyse my data.
03 April 2015 1,709 2 View
Hi, In my experiment I am interested to find interaction partner of the protein of my interest. For that I tagged my Protein with GFP and a pull down experiment was performed and then I analysed...
10 November 2014 2,069 4 View
Stable cell line.
09 October 2013 8,303 7 View
This is a very fundamental and practical problem faced by most of the lab working with molecular biology, mostly with RNA. I would like to discussion in this scientific forum regarding how most of...
08 September 2012 8,480 43 View
I want to pull down a membrane bound cytoplasmic GFP tagged protein. For this I ordered GFP tagged beads from chromotech. I am lysing the cells expressing GFP tagged protein of my interest with...
08 September 2012 5,310 5 View
I am working with the apoptosis inhibition by RNA virus. I could figure out the protein in my virus which decelerate the apoptosis in its host. I have also learnt from the literature that it uses...
04 May 2012 1,906 7 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View